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小鼠白介素10酶免試劑盒,(IL-10)ELISA檢測試劑盒 ,英文名:IL-10 ELISA Kit ,英文縮寫:IL-10 ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠白介素10ELISA檢測試劑盒,IL-10 檢測試劑盒, IL-10 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。小鼠白介素10酶免試劑盒,(IL-10)ELISA檢測試劑盒在操作中應(yīng)注意以下5個方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進(jìn)行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。嗅素4(OLFM4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Olfactomedin 4 (OLFM4)48T/96TY10765Bos taurus; Bovine (Cattle)
抑瘤素M(OSM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Oncostatin M (OSM)48T/96TY10766Rattus norvegicus (Rat)
肌肌酸激酶(CKM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Creatine Kinase, Muscle (CKM)48T/96TY10767Rattus norvegicus (Rat)
骨保護素(OPG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Osteoprotegerin (OPG)48T/96TY10768Rattus norvegicus (Rat)
膽囊收縮素A受體(CCKAR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cholecystokinin A Receptor (CCKAR)48T/96TY10769Rattus norvegicus (Rat)
基質(zhì)金屬蛋白酶8(MMP8)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Matrix Metalloproteinase 8 (MMP8)48T/96TY10770Rattus norvegicus (Rat)
基質(zhì)金屬蛋白酶8(MMP8)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Matrix Metalloproteinase 8 (MMP8)48T/96TY10771Mus musculus (Mouse)
基質(zhì)金屬蛋白酶7(MMP7)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Matrix Metalloproteinase 7 (MMP7)48T/96TY10772Rattus norvegicus (Rat)
基質(zhì)金屬蛋白酶7(MMP7)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Matrix Metalloproteinase 7 (MMP7)48T/96TY10773Mus musculus (Mouse)
基質(zhì)金屬蛋白酶3(MMP3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Matrix Metalloproteinase 3 (MMP3)48T/96TY10774Rattus norvegicus (Rat)
基質(zhì)金屬蛋白酶2(MMP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Matrix Metalloproteinase 2 (MMP2)48T/96TY10775Rattus norvegicus (Rat)
基質(zhì)金屬蛋白酶13(MMP13)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Matrix Metalloproteinase 13 (MMP13)48T/96TY10776Rattus norvegicus (Rat)
基質(zhì)金屬蛋白酶13(MMP13)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Matrix Metalloproteinase 13 (MMP13)48T/96TY10777Mus musculus (Mouse)
基質(zhì)金屬蛋白酶10(MMP10)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Matrix Metalloproteinase 10 (MMP10)48T/96TY10778Rattus norvegicus (Rat)
基質(zhì)金屬蛋白酶10(MMP10)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Matrix Metalloproteinase 10 (MMP10)48T/96TY10779Mus musculus (Mouse)
基質(zhì)金屬蛋白酶1(MMP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Matrix Metalloproteinase 1 (MMP1)48T/96TY10780Rattus norvegicus (Rat)
基質(zhì)金屬蛋白酶1(MMP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Matrix Metalloproteinase 1 (MMP1)48T/96TY10781Mus musculus (Mouse)
巨噬細(xì)胞炎性蛋白3β(MIP3β)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Macrophage Inflammatory Protein 3 Beta (MIP3b)48T/96TY10782Rattus norvegicus (Rat)
胱天蛋白酶11(CASP11)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Caspase 11 (CASP11)48T/96TY10783Rattus norvegicus (Rat)
胱天蛋白酶11(CASP11)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Caspase 11 (CASP11)48T/96TY10784Homo sapiens (Human)
巨噬細(xì)胞炎性蛋白1β(MIP1β)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Macrophage Inflammatory Protein 1 Beta (MIP1b)48T/96TY10785Rattus norvegicus (Rat)
巨噬細(xì)胞炎性蛋白1α(MIP1α)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Macrophage Inflammatory Protein 1 Alpha (MIP1a)48T/96TY10786Rattus norvegicus (Rat)
巨噬細(xì)胞來源趨化因子(MDC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Macrophage Derived Chemokine (MDC)48T/96TY10787Rattus norvegicus (Rat)
巨噬細(xì)胞集落刺激因子(MCSF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Colony Stimulating Factor 1, Macrophage (MCSF)48T/96TY10788Rattus norvegicus (Rat)
單核細(xì)胞趨化蛋白3(MCP3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Monocyte Chemotactic Protein 3 (MCP3)48T/96TY10789Rattus norvegicus (Rat)
單核細(xì)胞趨化蛋白3(MCP3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Monocyte Chemotactic Protein 3 (MCP3)48T/96TY10790Mus musculus (Mouse)
單核細(xì)胞趨化蛋白2(MCP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Monocyte Chemotactic Protein 2 (MCP2)48T/96TY10791Mus musculus (Mouse)
單核細(xì)胞趨化蛋白1(MCP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Monocyte Chemotactic Protein 1 (MCP1)48T/96TY10792Rattus norvegicus (Rat)
單核細(xì)胞趨化蛋白1(MCP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Monocyte Chemotactic Protein 1 (MCP1)48T/96TY10793Mus musculus (Mouse)
L選擇素(SELL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Selectin, Leukocyte (SELL)48T/96TY10794Rattus norvegicus (Rat)
白血病抑制因子(LIF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Leukemia Inhibitory Factor (LIF)48T/96TY10795Rattus norvegicus (Rat)
瘦素(LEP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Leptin (LEP)48T/96TY10796Rattus norvegicus (Rat)
瘦素受體(LEPR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Leptin Receptor (LEPR)48T/96TY10797Rattus norvegicus (Rat)
白介素5(IL5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 5 (IL5)48T/96TY10798Rattus norvegicus (Rat)
白介素3(IL3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 3 (IL3)48T/96TY10799Rattus norvegicus (Rat)
N-乙酰β-D-氨基葡萄糖苷酶(NAGase)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for N-Acetyl Beta-D-Glucosaminidase (NAGase)48T/96TY10800Rattus norvegicus (Rat)
N-乙酰β-D-氨基葡萄糖苷酶(NAGase)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for N-Acetyl Beta-D-Glucosaminidase (NAGase)48T/96TY10801Mus musculus (Mouse)
白介素1受體Ⅰ(IL1R1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 1 Receptor Type I (IL1R1)48T/96TY10802Rattus norvegicus (Rat)
白介素1受體Ⅰ(IL1R1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 1 Receptor Type I (IL1R1)48T/96TY10803Mus musculus (Mouse)
白介素1受體Ⅱ(IL1R2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 1 Receptor Type II (IL1R2)48T/96TY10804Rattus norvegicus (Rat)
白介素1受體Ⅱ(IL1R2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 1 Receptor Type II (IL1R2)48T/96TY10805Mus musculus (Mouse)
白介素18(IL18)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 18 (IL18)48T/96TY10806Homo sapiens (Human)
白介素17(IL17)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 17 (IL17)48T/96TY10807Rattus norvegicus (Rat)
白介素16(IL16)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 16 (IL16)48T/96TY10808Rattus norvegicus (Rat)
白介素15(IL15)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 15 (IL15)48T/96TY10809Rattus norvegicus (Rat)
白介素13(IL13)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 13 (IL13)48T/96TY10810Rattus norvegicus (Rat)
乙酰輔酶A羧化酶α(ACACα)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Acetyl Coenzyme A Carboxylase Alpha (ACACa)48T/96TY10811Rattus norvegicus (Rat)
促生長激素釋放激素(GHRH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Growth Hormone Releasing Hormone (GHRH)48T/96TY10812Homo sapiens (Human)
促生長激素釋放激素(GHRH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Growth Hormone Releasing Hormone (GHRH)48T/96TY10813Mus musculus (Mouse)
甲狀旁腺激素(PTH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Parathyroid Hormone (PTH)48T/96TY10814Rattus norvegicus (Rat)
甲狀旁腺激素(PTH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Parathyroid Hormone (PTH)48T/96TY10815Mus musculus (Mouse)
前列腺素E合酶2(PTGES2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Prostaglandin E Synthase 2 (PTGES2)48T/96TY10816Mus musculus (Mouse)
去甲腎上腺素(NE)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Noradrenaline (NE)48T/96TY10817General
結(jié)締組織生長因子(CTGF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Connective Tissue Growth Factor (CTGF)48T/96TY10818Homo sapiens (Human)
結(jié)締組織生長因子(CTGF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Connective Tissue Growth Factor (CTGF)48T/96TY10819Rattus norvegicus (Rat)
結(jié)締組織生長因子(CTGF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Connective Tissue Growth Factor (CTGF)48T/96TY10820Mus musculus (Mouse)
維生素D5(VD5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Vitamin D5 (VD5)48T/96TY10821Homo sapiens (Human)
組織因子(TF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tissue Factor (TF)48T/96TY10822Homo sapiens (Human)
組織因子(TF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tissue Factor (TF)48T/96TY10823Rattus norvegicus (Rat)
組織因子(TF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tissue Factor (TF)48T/96TY10824Mus musculus (Mouse)
組織型纖溶酶原激活因子(tPA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Plasminogen Activator, Tissue (tPA)48T/96TY10825Homo sapiens (Human)
各種類型ELISA測定時, 一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。