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小鼠IgG ELISA檢測試劑盒,英文名:小鼠IgG ELISA試劑盒,英文縮寫:小鼠IgG ELISA試劑盒,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠IgG ELISA試劑盒小鼠IgGELISA試劑盒
ELISA實驗操作較繁雜,操作不當將引起較大的誤差。小鼠IgG ELISA檢測試劑盒在操作中應注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內的含量發(fā)生大幅波動,因此有必要對標本進行預處理,例如對血清標本作適當稀釋,或離心分離血脂等。間接法測定中,標本適當稀釋還可降低非特異性反應。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標本、加酶結合物、加底物和加終止液。加標本時必須每份標本使用一支移液器吸嘴,以免交叉污染。加酶結合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應和酶促反應時間不*,對競爭法及定量檢測影響很大,不注意可能會導致錯誤結果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關鍵步驟。目的是除去未結合的免疫反應物,終止抗原抗體反應,除去標本中與反應無關的成分、游離的酶標物以及反應過程中吸附于固相載體上的非特異性物質??捎孟窗鍣C洗板或手工洗板,前者洗滌質量較好,各反應孔洗滌效果均一,批內、批間差異小,手工洗板應注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結果須使用ELISA測讀儀(酶標儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應孔的吸光度值(A值)。酶標儀具有良好的重復性。
磷脂酰肌醇蛋白聚糖4(GPC4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glypican 4 (GPC4)48T/96TY11685Mus musculus (Mouse)
干擾素調節(jié)因子3(IRF3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interferon Regulatory Factor 3 (IRF3)48T/96TY11686Mus musculus (Mouse)
細胞外基質蛋白1(ECM1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Extracellular Matrix Protein 1 (ECM1)48T/96TY11687Homo sapiens (Human)
腎上腺髓質素(ADM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Adrenomedullin (ADM)48T/96TY11688Equus caballus; Equine (Horse)
干擾素β(IFNβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interferon Beta (IFNb)48T/96TY11689Rhesus monkey (Simian)
白介素1受體拮抗劑(IL1RA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 1 Receptor Antagonist (IL1RA)48T/96TY11690Canis familiaris; Canine (Dog)
白介素1受體拮抗劑(IL1RA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 1 Receptor Antagonist (IL1RA)48T/96TY11691Capra hircus; Caprine (Goat)
白介素1受體拮抗劑(IL1RA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 1 Receptor Antagonist (IL1RA)48T/96TY11692Equus caballus; Equine (Horse)
白介素1受體拮抗劑(IL1RA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 1 Receptor Antagonist (IL1RA)48T/96TY11693Oryctolagus cuniculus (Rabbit)
干擾素β(IFNβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interferon Beta (IFNb)48T/96TY11694Equus caballus; Equine (Horse)
基質金屬蛋白酶11(MMP11)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Matrix Metalloproteinase 11 (MMP11)48T/96TY11695Rattus norvegicus (Rat)
白介素1受體拮抗劑(IL1RA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 1 Receptor Antagonist (IL1RA)48T/96TY11696Bos taurus; Bovine (Cattle)
白介素18(IL18)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 18 (IL18)48T/96TY11697Capra hircus; Caprine (Goat)
白介素18(IL18)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 18 (IL18)48T/96TY11698Equus caballus; Equine (Horse)
白介素1受體拮抗劑(IL1RA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 1 Receptor Antagonist (IL1RA)48T/96TY11699Rhesus monkey (Simian)
白介素18(IL18)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 18 (IL18)48T/96TY11700Oryctolagus cuniculus (Rabbit)
心鈉肽(ANP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Atrial Natriuretic Peptide (ANP)48T/96TY11701Bos taurus; Bovine (Cattle)
心鈉肽(ANP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Atrial Natriuretic Peptide (ANP)48T/96TY11702Equus caballus; Equine (Horse)
心鈉肽(ANP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Atrial Natriuretic Peptide (ANP)48T/96TY11703Cavia (Guinea pig )
心鈉肽(ANP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Atrial Natriuretic Peptide (ANP)48T/96TY11704Sus scrofa; Porcine (Pig)
白介素1受體Ⅱ(IL1R2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 1 Receptor Type II (IL1R2)48T/96TY11705Rhesus monkey (Simian)
末端補體復合體C5b-9(C5b-9)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Terminal Complement Complex C5b-9 (C5b-9)48T/96TY11706Mus musculus (Mouse)
神經(jīng)膠質纖維酸性蛋白(GFAP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glial Fibrillary Acidic Protein (GFAP)48T/96TY11707Bos taurus; Bovine (Cattle)
激活素B(ACVB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Activin B (ACVB)48T/96TY11708Homo sapiens (Human)
Ⅲ型膠原(COL3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Collagen Type III (COL3)48T/96TY11709Bos taurus; Bovine (Cattle)
α2-HS糖蛋白(αHSG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Alpha-2-Heremans Schmid Glycoprotein (aHSG)48T/96TY11710Bos taurus; Bovine (Cattle)
α2-HS糖蛋白(αHSG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Alpha-2-Heremans Schmid Glycoprotein (aHSG)48T/96TY11711Capra hircus; Caprine (Goat)
α2-HS糖蛋白(αHSG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Alpha-2-Heremans Schmid Glycoprotein (aHSG)48T/96TY11712Cavia (Guinea pig )
血小板因子4(PF4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Plaet Factor 4 (PF4)48T/96TY11713Bos taurus; Bovine (Cattle)
血小板因子4(PF4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Plaet Factor 4 (PF4)48T/96TY11714Capra hircus; Caprine (Goat)
血小板因子4(PF4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Plaet Factor 4 (PF4)48T/96TY11715Sus scrofa; Porcine (Pig)
CD99分子(CD99)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cluster Of Differentiation 99 (CD99)48T/96TY11716Homo sapiens (Human)
α2-HS糖蛋白(αHSG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Alpha-2-Heremans Schmid Glycoprotein (aHSG)48T/96TY11717Oryctolagus cuniculus (Rabbit)
α2-HS糖蛋白(αHSG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Alpha-2-Heremans Schmid Glycoprotein (aHSG)48T/96TY11718Rhesus monkey (Simian)
半乳糖苷酶α(GLα)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Galactosidase Alpha (GLa)48T/96TY11719Homo sapiens (Human)
半乳糖苷酶α(GLα)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Galactosidase Alpha (GLa)48T/96TY11720Mus musculus (Mouse)
中性粒細胞彈性蛋白酶(ELA2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Elastase 2, Neutrophil (ELA2)48T/96TY11721Canis familiaris; Canine (Dog)
胃蛋白酶原A(PGA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Pepsinogen A (PGA)48T/96TY11722Bos taurus; Bovine (Cattle)
胃蛋白酶原A(PGA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Pepsinogen A (PGA)48T/96TY11723Mus musculus (Mouse)
胃蛋白酶原C(PGC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Pepsinogen C (PGC)48T/96TY11724Chicken (Gallus)
胃蛋白酶原C(PGC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Pepsinogen C (PGC)48T/96TY11725Mus musculus (Mouse)
泛素(Ub)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ubiquitin (Ub)48T/96TY11726Bos taurus; Bovine (Cattle)
泛素(Ub)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ubiquitin (Ub)48T/96TY11727Canis familiaris; Canine (Dog)
泛素(Ub)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ubiquitin (Ub)48T/96TY11728Equus caballus; Equine (Horse)
泛素(Ub)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ubiquitin (Ub)48T/96TY11729Chicken (Gallus)
泛素(Ub)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ubiquitin (Ub)48T/96TY11730Cavia (Guinea pig )
泛素(Ub)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ubiquitin (Ub)48T/96TY11731Sus scrofa; Porcine (Pig)
泛素(Ub)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ubiquitin (Ub)48T/96TY11732Oryctolagus cuniculus (Rabbit)
泛素(Ub)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ubiquitin (Ub)48T/96TY11733Rhesus monkey (Simian)
血管生長素(ANG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Angiogenin (ANG)48T/96TY11734Bos taurus; Bovine (Cattle)
各種類型ELISA測定時, 一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結果。