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小鼠絲氨酸/蘇氨酸蛋白磷酸酶酶免試劑盒,(STK)ELISA檢測試劑盒 ,英文名:STK ELISA Kit ,英文縮寫:STK ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠絲氨酸/蘇氨酸蛋白磷酸酶ELISA檢測試劑盒,STK 檢測試劑盒, STK 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。小鼠絲氨酸/蘇氨酸蛋白磷酸酶酶免試劑盒,(STK)ELISA檢測試劑盒在操作中應(yīng)注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
成纖維細胞生長因子受體3(FGFR3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibroblast Growth Factor Receptor 3 (FGFR3)48T/96TY12485Homo sapiens (Human)
成纖維細胞生長因子受體4(FGFR4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibroblast Growth Factor Receptor 4 (FGFR4)48T/96TY12486Homo sapiens (Human)
皮膚衍生肽酶抑制因子3(PI3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Peptidase Inhibitor 3, Skin Derived (PI3)48T/96TY12487Homo sapiens (Human)
Ⅶ型膠原(COL7)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Collagen Type VII (COL7)48T/96TY12488Homo sapiens (Human)
白介素17C(IL17C)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 17C (IL17C)48T/96TY12489Mus musculus (Mouse)
轉(zhuǎn)鐵蛋白受體(TFR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transferrin Receptor (TFR)48T/96TY12490Rattus norvegicus (Rat)
整合素β3(ITGβ3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Integrin Beta 3 (ITGb3)48T/96TY12491Homo sapiens (Human)
腫瘤壞死因子配體超家族成員14(TNFSF14)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tumor Necrosis Factor Ligand Superfamily, Member 14 (TNFSF14)48T/96TY12492Mus musculus (Mouse)
基質(zhì)金屬蛋白酶19(MMP19)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Matrix Metalloproteinase 19 (MMP19)48T/96TY12493Mus musculus (Mouse)
骨形成蛋白8B(BMP8B)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Bone Morphogenetic Protein 8B (BMP8B)48T/96TY12494Mus musculus (Mouse)
胰島素樣蛋白5(INSL5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Insulin Like Protein 5 (INSL5)48T/96TY12495Mus musculus (Mouse)
N-乙酰α-D-氨基葡萄糖苷酶(NAGLU)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for N-Acetyl Alpha-D-Glucosaminidase (NAGLU)48T/96TY12496Homo sapiens (Human)
尿皮質(zhì)素2(UCN2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Urocortin 2 (UCN2)48T/96TY12497Homo sapiens (Human)
層粘連蛋白γ2(LAMγ2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Laminin Gamma 2 (LAMC2)48T/96TY12498Homo sapiens (Human)
鈣周期素結(jié)合蛋白(CACYBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Calcyclin Binding Protein (CACYBP)48T/96TY12499Homo sapiens (Human)
癌胚抗原相關(guān)細胞粘附分子1(CEACAM1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Carcinoembryonic Antigen Related Cell Adhesion Molecule 1 (CEACAM1)48T/96TY12500Homo sapiens (Human)
胰腺型磷脂酶A2(pPLA2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Phospholipase A2, Pancreas (pPLA2)48T/96TY12501Sus scrofa; Porcine (Pig)
Corin蛋白(CRN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Corin (CRN)48T/96TY12502Homo sapiens (Human)
Rho GDP解離抑制因子α(ARHGDIα)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Rho GDP Dissociation Inhibitor Alpha (ARHGDIa)48T/96TY12503Homo sapiens (Human)
胞裂蛋白5(SEPT5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Septin 5 (SEPT5)48T/96TY12504Homo sapiens (Human)
胃動素(MTL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Motilin (MTL)48T/96TY12505Rattus norvegicus (Rat)
組織轉(zhuǎn)谷氨酰胺酶(TGM2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transglutaminase 2, Tissue (TGM2)48T/96TY12506Rattus norvegicus (Rat)
補體成分1r(C1r)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Complement Component 1, R Subcomponent (C1r)48T/96TY12507Homo sapiens (Human)
補體成分1s(C1s)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Complement Component 1, S Subcomponent (C1s)48T/96TY12508Homo sapiens (Human)
微管關(guān)聯(lián)絲/蘇氨酸激酶2(MAST2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Microtubule Associated Serine/Threonine Kinase 2 (MAST2)48T/96TY12509Homo sapiens (Human)
快肌肌鈣蛋白C(TNNC2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Troponin C Type 2, Fast (TNNC2)48T/96TY12510Homo sapiens (Human)
彈性蛋白(ELN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Elastin (ELN)48T/96TY12511Mus musculus (Mouse)
丙酮酸脫氫酶β(PDHβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Pyruvate Dehydrogenase Beta (PDHb)48T/96TY12512Mus musculus (Mouse)
賴氨酸t(yī)RNA合成酶(KARS)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lysyl tRNA Synthetase (KARS)48T/96TY12513Homo sapiens (Human)
賴氨酸t(yī)RNA合成酶(KARS)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lysyl tRNA Synthetase (KARS)48T/96TY12514Mus musculus (Mouse)
酮戊二酸脫氫酶(OGDH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Oxoglutarate Dehydrogenase (OGDH)48T/96TY12515Homo sapiens (Human)
鈣網(wǎng)蛋白(CRT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Calreticulin (CRT)48T/96TY12516Mus musculus (Mouse)
CD83分子(CD83)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cluster Of Differentiation 83 (CD83)48T/96TY12517Homo sapiens (Human)
組織蛋白酶B(CTSB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cathepsin B (CTSB)48T/96TY12518Homo sapiens (Human)
外核苷酸焦磷酸酶/磷酸二酯酶2(ENPP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ectonucleotide Pyrophosphatase/Phosphodiesterase 2 (ENPP2)48T/96TY12519Homo sapiens (Human)
白介素18結(jié)合蛋白(IL18BP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 18 Binding Protein (IL18BP)48T/96TY12520Mus musculus (Mouse)
卵磷脂膽固醇脂酰轉(zhuǎn)移酶(LCAT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lecithin Cholesterol Acyltransferase (LCAT)48T/96TY12521Homo sapiens (Human)
支鏈α-酮酸脫氫酶(BCKDK)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Branched Chain Alpha-Ketoacid Dehydrogenase Kinase (BCKDK)48T/96TY12522Rattus norvegicus (Rat)
白介素19(IL19)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 19 (IL19)48T/96TY12523Homo sapiens (Human)
花生四烯酸-5-脂加氧酶(ALOX5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Arachidonate-5-Lipoxygenase (ALOX5)48T/96TY12524Mus musculus (Mouse)
血管生成素樣蛋白2(ANGPTL2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Angiopoietin Like Protein 2 (ANGPTL2)48T/96TY12525Mus musculus (Mouse)
細胞色素P450氧化還原酶(CPR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cytochrome P450 Reductase (CPR)48T/96TY12526Rattus norvegicus (Rat)
C-型凝集素域家族3成員B(CLEC3B)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for C-Type Lectin Domain Family 3, Member B (CLEC3B)48T/96TY12527Homo sapiens (Human)
神經(jīng)元正五聚蛋白Ⅱ(NPTX2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Neuronal Pentraxin II (NPTX2)48T/96TY12528Homo sapiens (Human)
免疫球蛋白G(IgG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Immunoglobulin G (IgG)48T/96TY12529Bos taurus; Bovine (Cattle)
促胰液素(SCT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Secretin (SCT)48T/96TY12530Mus musculus (Mouse)
骨成型蛋白10(BMP10)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Bone Morphogenetic Protein 10 (BMP10)48T/96TY12531Homo sapiens (Human)
自身免疫調(diào)節(jié)因子(AIRE)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Autoimmune Regulator (AIRE)48T/96TY12532Homo sapiens (Human)
5羥色胺轉(zhuǎn)運蛋白(SERT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Serotonin Transporter (SERT)48T/96TY12533Homo sapiens (Human)
組織蛋白酶G(CTSG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cathepsin G (CTSG)48T/96TY12534Rattus norvegicus (Rat)
各種類型ELISA測定時,一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。