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小鼠粘蛋白/粘液素7酶免試劑盒,(MUC7)ELISA檢測試劑盒 ,英文名:MUC7 ELISA Kit ,英文縮寫:MUC7 ,常見試劑盒規(guī)格:96T/48T,產品別名:小鼠粘蛋白/粘液素7ELISA檢測試劑盒,MUC7 檢測試劑盒, MUC7 檢測試劑盒
ELISA實驗操作較繁雜,操作不當將引起較大的誤差。小鼠粘蛋白/粘液素7酶免試劑盒,(MUC7)ELISA檢測試劑盒在操作中應注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內的含量發(fā)生大幅波動,因此有必要對標本進行預處理,例如對血清標本作適當稀釋,或離心分離血脂等。間接法測定中,標本適當稀釋還可降低非特異性反應。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標本、加酶結合物、加底物和加終止液。加標本時必須每份標本使用一支移液器吸嘴,以免交叉污染。加酶結合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應和酶促反應時間不*,對競爭法及定量檢測影響很大,不注意可能會導致錯誤結果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關鍵步驟。目的是除去未結合的免疫反應物,終止抗原抗體反應,除去標本中與反應無關的成分、游離的酶標物以及反應過程中吸附于固相載體上的非特異性物質??捎孟窗鍣C洗板或手工洗板,前者洗滌質量較好,各反應孔洗滌效果均一,批內、批間差異小,手工洗板應注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結果須使用ELISA測讀儀(酶標儀),比色法判讀可選擇單波長或雙波長,根據反應液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應孔的吸光度值(A值)。酶標儀具有良好的重復性。
乳腺絲抑蛋白(Maspin)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Maspin48T/96TY13135Homo sapiens (Human)
幾丁質酶1(CHIT1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Chitinase 1 (CHIT1)48T/96TY13136Mus musculus (Mouse)
Zeste同源物增強子2(EZH2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Enhancer Of Zeste Homolog 2 (EZH2)48T/96TY13137Homo sapiens (Human)
微小染色體維持缺陷蛋白5(MCM5)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Minichromosome Maintenance Deficient 5 (MCM5)48T/96TY13138Homo sapiens (Human)
肝配蛋白A4(EFNA4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Ephrin A4 (EFNA4)48T/96TY13139Homo sapiens (Human)
EB病毒誘導蛋白3(EBI3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Epstein Barr Virus Induced Protein 3 (EBI3)48T/96TY13140Mus musculus (Mouse)
前妊娠蛋白關聯子宮內膜蛋白(PAEP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Progestagen Associated Endometrial Protein (PAEP)48T/96TY13141Homo sapiens (Human)
淋巴細胞抗原75(LY75)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Lymphocyte Antigen 75 (LY75)48T/96TY13142Mus musculus (Mouse)
CD276分子(CD276)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cluster Of Differentiation 276 (CD276)48T/96TY13143Homo sapiens (Human)
信號素3E(SEMA3E)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Semaphorin 3E (SEMA3E)48T/96TY13144Mus musculus (Mouse)
小白蛋白(PVALB)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Parvalbumin (PVALB)48T/96TY13145Rattus norvegicus (Rat)
衰老關鍵蛋白4(FBLN4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fibulin 4 (FBLN4)48T/96TY13146Homo sapiens (Human)
Sideroflexin 1蛋白(SFXN1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Sideroflexin 1 (SFXN1)48T/96TY13147Mus musculus (Mouse)
SRC/FGR/YES相關FYN癌基因(FYN)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for FYN Oncogene Related To SRC/FGR/YES (FYN)48T/96TY13148Mus musculus (Mouse)
染色質裝配因子1亞基B(CHAF1B)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Chromatin Assembly Factor 1, Subunit B (CHAF1B)48T/96TY13149Mus musculus (Mouse)
氨酰tRNA合成酶復合多功能相互作用蛋白1(AIMP1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1)48T/96TY13150Homo sapiens (Human)
氨酰tRNA合成酶復合多功能相互作用蛋白1(AIMP1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1)48T/96TY13151Mus musculus (Mouse)
堿性唾液脯氨酸豐富蛋白2(PRB2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Basic Salivary Proline Rich Protein 2 (PRB2)48T/96TY13152Homo sapiens (Human)
堿性唾液脯氨酸豐富蛋白1(PRB1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Basic Salivary Proline Rich Protein 1 (PRB1)48T/96TY13153Homo sapiens (Human)
Dickkopf相關蛋白4(DKK4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Dickkopf Related Protein 4 (DKK4)48T/96TY13154Homo sapiens (Human)
Dickkopf相關蛋白4(DKK4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Dickkopf Related Protein 4 (DKK4)48T/96TY13155Mus musculus (Mouse)
天冬氨酸β羥化酶(ASPH)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Aspartate Beta Hydroxylase (ASPH)48T/96TY13156Homo sapiens (Human)
分泌型卷曲相關蛋白2(SFRP2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Secreted Frizzled Related Protein 2 (SFRP2)48T/96TY13157Homo sapiens (Human)
序列相似家族3成員B(FAM3B)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Family With Sequence Similarity 3, Member B (FAM3B)48T/96TY13158Mus musculus (Mouse)
含膠原三螺旋重復蛋白1(CTHRC1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Collagen Triple Helix Repeat Containing Protein 1 (CTHRC1)48T/96TY13159Homo sapiens (Human)
AE結合蛋白1(AEBP1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for AE Binding Protein 1 (AEBP1)48T/96TY13160Homo sapiens (Human)
微管蛋白β1(TUBβ1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Tubulin Beta 1 (TUBb1)48T/96TY13161Mus musculus (Mouse)
酶原顆粒膜糖蛋白(GP2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Glycoprotein 2, Zymogen Granule Membrane (GP2)48T/96TY13162Rattus norvegicus (Rat)
花生四烯酸脂加氧酶3(ALOXE3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Arachidonate Lipoxygenase 3 (ALOXE3)48T/96TY13163Homo sapiens (Human)
癌胚抗原相關細胞粘附分子6(CEACAM6)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Carcinoembryonic Antigen Related Cell Adhesion Molecule 6 (CEACAM6)48T/96TY13164Homo sapiens (Human)
線粒體解偶聯蛋白1(UCP1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Uncoupling Protein 1, Mitochondrial (UCP1)48T/96TY13165Rattus norvegicus (Rat)
F-框亮氨酸豐富重復蛋白3(FBXL3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for F-Box And Leucine Rich Repeat Protein 3 (FBXL3)48T/96TY13166Mus musculus (Mouse)
脂肪酸合酶(FASN)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fatty Acid Synthase (FASN)48T/96TY13167Mus musculus (Mouse)
金屬硫蛋白1M(MT1M)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Metallothionein 1M (MT1M)48T/96TY13168Homo sapiens (Human)
Vasorin蛋白(VASN)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Vasorin (VASN)48T/96TY13169Homo sapiens (Human)
精胺氧化酶(SMOX)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Spermine Oxidase (SMOX)48T/96TY13170Mus musculus (Mouse)
絲氨酸棕櫚酰轉移酶長鏈堿性亞基1(SPTLC1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1)48T/96TY13171Mus musculus (Mouse)
基質金屬蛋白酶9(MMP9)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Matrix Metalloproteinase 9 (MMP9)48T/96TY13172Canis familiaris; Canine (Dog)
5羥色胺轉運蛋白(SERT)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Serotonin Transporter (SERT)48T/96TY13173Rattus norvegicus (Rat)
3-羥基3-甲基戊二酰輔酶A還原酶(HMGCR)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR)48T/96TY13174Rattus norvegicus (Rat)
鈣調蛋白樣蛋白5(CALML5)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Calmodulin Like Protein 5 (CALML5)48T/96TY13175Homo sapiens (Human)
軟骨關聯蛋白(CRTAP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cartilage Associated Protein (CRTAP)48T/96TY13176Mus musculus (Mouse)
AF4/FMR2家族成員1(AFF1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for AF4/FMR2 Family, Member 1 (AFF1)48T/96TY13177Homo sapiens (Human)
白介素1家族成員9(IL1F9)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interleukin 1 Family, Member 9 (IL1F9)48T/96TY13178Mus musculus (Mouse)
基質金屬蛋白酶2(MMP2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Matrix Metalloproteinase 2 (MMP2)48T/96TY13179Sus scrofa; Porcine (Pig)
次黃嘌呤磷酸核糖基轉移酶1(HPRT1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Hypoxanthine Phosphoribosyltransferase 1 (HPRT1)48T/96TY13180Homo sapiens (Human)
WNT抑制因子1(WIF1)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for WNT Inhibitory Factor 1 (WIF1)48T/96TY13181Homo sapiens (Human)
腎足蛋白(NPHN)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Nephrin (NPHN)48T/96TY13182Rattus norvegicus (Rat)
CD200分子(CD200)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cluster Of Differentiation 200 (CD200)48T/96TY13183Homo sapiens (Human)
干擾素α/β受體2(IFNα/βR2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interferon Alpha/Beta Receptor 2 (IFNa/bR2)48T/96TY13184Homo sapiens (Human)
各種類型ELISA測定時, 一般需經過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結果。