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小鼠D-乳酸脫氫酶酶免試劑盒,(D-LDH)ELISA檢測試劑盒 ,英文名:D-LDH ELISA Kit ,英文縮寫:D-LDH ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠D-乳酸脫氫酶ELISA檢測試劑盒,D-LDH 檢測試劑盒, D-LDH 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。小鼠D-乳酸脫氫酶酶免試劑盒,(D-LDH)ELISA檢測試劑盒 在操作中應(yīng)注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
TNF受體關(guān)聯(lián)因子6(TRAF6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for TNF Receptor Associated Factor 6 (TRAF6)48T/96TY13285Homo sapiens (Human)
旁血小板溶蛋白(PPL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Periplakin (PPL)48T/96TY13286Mus musculus (Mouse)
淋巴細胞抗原96(LY96)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lymphocyte Antigen 96 (LY96)48T/96TY13287Homo sapiens (Human)
Diaphanous同源物1(DIAPH1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Diaphanous Homolog 1 (DIAPH1)48T/96TY13288Homo sapiens (Human)
解整合素金屬蛋白酶22(ADAM22)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for A Disintegrin And Metalloprotease 22 (ADAM22)48T/96TY13289Homo sapiens (Human)
電子轉(zhuǎn)移黃素蛋白α肽(ETFα)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Electron Transfer Flavoprotein Alpha Polypeptide (ETFa)48T/96TY13290Homo sapiens (Human)
精氨基琥珀酸合成酶1(ASS1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Argininosuccinate Synthetase 1 (ASS1)48T/96TY13291Homo sapiens (Human)
蛋白二硫化物異構(gòu)酶A6(PDIA6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein Disulfide Isomerase A6 (PDIA6)48T/96TY13292Homo sapiens (Human)
半胱氨酸蛋白酶抑制劑6(CST6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cystatin 6 (CST6)48T/96TY13293Homo sapiens (Human)
促泌素(SCGN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Secretagogin (SCGN)48T/96TY13294Rattus norvegicus (Rat)
促泌素(SCGN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Secretagogin (SCGN)48T/96TY13295Mus musculus (Mouse)
賴氨酰氧化酶樣蛋白4(LOXL4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lysyl Oxidase Like Protein 4 (LOXL4)48T/96TY13296Homo sapiens (Human)
內(nèi)皮細胞特異分子1(ESM1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Endothelial Cell Specific Molecule 1 (ESM1)48T/96TY13297Homo sapiens (Human)
金屬硫蛋白1E(MT1E)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Metallothionein 1E (MT1E)48T/96TY13298Homo sapiens (Human)
凝血因子Ⅵ(F6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Coagulation Factor VI (F6)48T/96TY13299Rattus norvegicus (Rat)
無翅型MMTV整合位點家族成員4(WNT4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Wingless Type MMTV Integration Site Family, Member 4 (WNT4)48T/96TY13300Rattus norvegicus (Rat)
USP6氨基端樣蛋白(USP6NL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for USP6 N-Terminal Like Protein (USP6NL)48T/96TY13301Homo sapiens (Human)
血幼素(HJV)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Hemojuvelin (HJV)48T/96TY13302Rattus norvegicus (Rat)
鹽皮質(zhì)激素受體(MR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Mineralocorticoid Receptor (MR)48T/96TY13303Rattus norvegicus (Rat)
AMPA離子能谷氨酸受體1(GRIA1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glutamate Receptor, Ionotropic, AMPA 1 (GRIA1)48T/96TY13304Rattus norvegicus (Rat)
N-甲基-D-天氡氨酸離子能谷氨酸受體1(GRIN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1)48T/96TY13305Rattus norvegicus (Rat)
殺菌性/通透性增加蛋白(BPI)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Bactericidal/Permeability Increasing Protein (BPI)48T/96TY13306Mus musculus (Mouse)
骨骼肌肌動蛋白α1(ACTα1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Actin Alpha 1, Skeletal Muscle (ACTa1)48T/96TY13307Rattus norvegicus (Rat)
骨骼肌慢肌肌鈣蛋白T(TNNT1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Troponin T Type 1, Slow Skeletal (TNNT1)48T/96TY13308Homo sapiens (Human)
細胞色素P450家族成員1B1(CYP1B1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cytochrome P450 1B1 (CYP1B1)48T/96TY13309Homo sapiens (Human)
肌球蛋白重鏈6(MYH6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6)48T/96TY13310Rattus norvegicus (Rat)
絲裂原激活蛋白激酶激酶1(MAP2K1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Mitogen Activated Protein Kinase Kinase 1 (MAP2K1)48T/96TY13311Homo sapiens (Human)
血小板反應(yīng)蛋白2(THBS2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Thrombospondin 2 (THBS2)48T/96TY13312Homo sapiens (Human)
肝配蛋白A受體10(EPHA10)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ephrin Type A Receptor 10 (EPHA10)48T/96TY13313Homo sapiens (Human)
轉(zhuǎn)化受體電位陽離子通道亞家族M成員6(TRPM6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transient Receptor Potential Cation Channel Subfamily M, Member 6 (TRPM6)48T/96TY13314Rattus norvegicus (Rat)
轉(zhuǎn)化受體電位陽離子通道亞家族M成員7(TRPM7)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transient Receptor Potential Cation Channel Subfamily M, Member 7 (TRPM7)48T/96TY13315Rattus norvegicus (Rat)
轉(zhuǎn)化受體電位陽離子通道亞家族V成員1(TRPV1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transient Receptor Potential Cation Channel Subfamily V, Member 1 (TRPV1)48T/96TY13316Mus musculus (Mouse)
色胺酸羥化酶2(TPH2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tryptophan Hydroxylase 2 (TPH2)48T/96TY13317Mus musculus (Mouse)
RNA聚合酶轉(zhuǎn)錄終止因子Ⅰ(TTF1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transcription Termination Factor, RNA polymerase I (TTF1)48T/96TY13318Homo sapiens (Human)
Podocan蛋白(PODN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Podocan (PODN)48T/96TY13319Mus musculus (Mouse)
生長因子受體結(jié)合蛋白10(Grb10)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Growth Factor Receptor Bound Protein 10 (Grb10)48T/96TY13320Homo sapiens (Human)
胰島素樣生長因子2-mRNA結(jié)合蛋白3(IGF2BP3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3)48T/96TY13321Homo sapiens (Human)
黏病毒耐藥蛋白1(MX1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Myxovirus Resistance 1 (MX1)48T/96TY13322Homo sapiens (Human)
酶原粒蛋白16同源物B(ZG16B)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Zymogen Granule Protein 16 Homolog B (ZG16B)48T/96TY13323Homo sapiens (Human)
甘露糖受體C1樣蛋白1(MRC1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Mannose Receptor C Type 1 Like Protein 1(MRC1)48T/96TY13324Homo sapiens (Human)
糖蛋白Ⅴ(GP5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glycoprotein V, Plaet (GP5)48T/96TY13325Homo sapiens (Human)
白介素10受體α(IL10Rα)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 10 Receptor Alpha (IL10Ra)48T/96TY13326Rattus norvegicus (Rat)
白介素10受體β(IL10Rβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 10 Receptor Beta (IL10Rb)48T/96TY13327Rattus norvegicus (Rat)
巢蛋白(NID)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nidogen (NID)48T/96TY13328Homo sapiens (Human)
靜止素Q6硫基氧化酶1(QSOX1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1)48T/96TY13329Homo sapiens (Human)
連接橋粒斑珠蛋白(JUP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Junction Plakoglobin (JUP)48T/96TY13330Homo sapiens (Human)
抗增生抗體靶分子1(TAPA1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Target Of The Antiproliferative Antibody 1 (TAPA1)48T/96TY13331Homo sapiens (Human)
細胞外基質(zhì)磷酸糖蛋白(MEPE)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Matrix Extracellular Phosphoglycoprotein (MEPE)48T/96TY13332Mus musculus (Mouse)
苯酚磺基轉(zhuǎn)移酶(PST)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Phenol Sulfotransferase (PST)48T/96TY13333Mus musculus (Mouse)
凝血因子Ⅶ(F7)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Coagulation Factor VII (F7)48T/96TY13334Mus musculus (Mouse)
各種類型ELISA測定時, 一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。