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大鼠解整合素樣金屬蛋白酶8酶免試劑盒,(ADAM8)ELISA檢測試劑盒,英文名:ADAM8 ELISA Kit,英文縮寫:ADAM8 ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:大鼠解整合素樣金屬蛋白酶8ELISA檢測試劑盒,ADAM8 檢測試劑盒,ADAM8 檢測試劑盒
ELISA實(shí)驗(yàn)操作較繁雜,操作不當(dāng)將引起較大的誤差。大鼠解整合素樣金屬蛋白酶8酶免試劑盒,(ADAM8)ELISA檢測試劑盒在操作中應(yīng)注意以下5個(gè)方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機(jī)體內(nèi)的含量發(fā)生大幅波動(dòng),因此有必要對標(biāo)本進(jìn)行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時(shí)必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時(shí)則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時(shí)差的影響。加樣及混勻時(shí)間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時(shí)間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯(cuò)誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實(shí)驗(yàn)成敗的一個(gè)關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C(jī)洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性 人可溶性腫瘤壞死因子相關(guān)凋亡誘導(dǎo)配體(sTRAIL)ELISA試劑盒 Human soluble tumor necrosis factor-related apoptosis inducing ligand,sTRAIL ELISA Kit Y3361996T/48T
人腫瘤壞死因子相關(guān)凋亡誘導(dǎo)配體4(TRAIL-R4)ELISA試劑盒Human tumor necrosis factor-related apoptosis-inducing ligand 4,TRAIL-R4 ELISA KitY3362096T/48T
人腫瘤壞死因子相關(guān)凋亡誘導(dǎo)配體3(TRAIL-R3)ELISA試劑盒Human tumor necrosis factor-related apoptosis-inducing ligand 3,TRAIL-R3 ELISA KitY3362196T/48T
人腫瘤壞死因子相關(guān)凋亡誘導(dǎo)配體1(TRAIL-R1)ELISA試劑盒Human tumor necrosis factor-related apoptosis-inducing ligand 1,TRAIL-R1 ELISA KitY3362296T/48T
人腫瘤壞死因子可溶性受體Ⅱ(TNFsR-Ⅱ)ELISA試劑盒Human Tumor necrosis factor soluble receptor Ⅱ,TNFsR-Ⅱ ELISA Kit Y3362396T/48T
人腫瘤壞死因子可溶性受體Ⅰ(TNFsR-Ⅰ)ELISA試劑盒Human Tumor necrosis factor soluble receptor Ⅰ,TNFsR-ⅠELISA Kit Y3362496T/48T
人基質(zhì)細(xì)胞衍生因子1β(SDF-1β/CXCL12)ELISA試劑盒 Human Stromal cell derived factor 1β,SDF-1β ELISA KitY3362596T/48T
人干細(xì)胞因子受體(SCFR)ELISA試劑盒Human Stem Cell Factor Receptor,SCFR ELISA Kit Y3362696T/48T
人正常T細(xì)胞表達(dá)和分泌因子(RANTES/CCL5)ELISA試劑盒 Human regulated on activation in normal T-cell expressed and secreted,RANTES ELISA Kit Y3362796T/48T
人神經(jīng)營養(yǎng)因子4(NT-4)ELISA試劑盒Human Neurotrophin 4,NT-4 ELISA Kit Y3362896T/48T
人神經(jīng)營養(yǎng)因子3(NT-3)ELISA試劑盒人神經(jīng)營養(yǎng)因子3(NT-3)ELISA KitY3362996T/48T
人的神經(jīng)生長因子(NGF)ELISA試劑盒 Human Nerve growth factor,NGF ELISA Kit Y3363096T/48T
人巨噬細(xì)胞炎性蛋白3β(MIP-3β/ELC/CCL19)ELISA試劑盒Human Macrophage Inflammatory Protein-3β,MIP-3β ELISA Kit Y3363196T/48T
人巨噬細(xì)胞炎性蛋白3α(MIP-3α/CCL20)ELISA試劑盒Human Macrophage Inflammatory Protein-3α,MIP-3α ELISA Kit Y3363296T/48T
人巨噬細(xì)胞炎性蛋白1β(MIP-1β/CCL4)ELISA試劑盒 Human Macrophage Inflammatory Protein-1β,MIP-1β ELISA KitY3363396T/48T
人巨噬細(xì)胞炎性蛋白1α(MIP-1α/CCL3)ELISA試劑盒Human Macrophage Inflammatory Protein-1α,MIP-1α ELISA Kit Y3363496T/48T
人單核細(xì)胞趨化蛋白3(MCP-3/CCL7)ELISA試劑盒Human monocyte chemotactic protein 3,MCP-3 ELISA KitY3363596T/48T
人單核細(xì)胞趨化蛋白2(MCP-2/CCL8)ELISA試劑盒Human monocyte chemotactic protein 2,MCP-2 ELISA Kit Y3363696T/48T
人單核細(xì)胞趨化蛋白1(MCP-1/CCL2/MCAF)ELISA試劑盒Human monocyte chemotactic protein 1/monocyte chemotactic and activating factor,MCP-1/MCAF ELISA KitY3363796T/48T
人胰島素樣生長因子結(jié)合蛋白3(IGFBP-3)ELISA試劑盒Human insulin-like growth factors binding protein 3,IGFBP-3 ELISA Kit Y3363896T/48T
人胰島素樣生長因子結(jié)合蛋白2(IGFBP2)ELISA試劑盒Human insulin-like growth factors binding protein 2,IGFBP-2 ELISA Kit Y3363996T/48T
人胰島素樣生長因子結(jié)合蛋白1(IGFBP-1)ELISA試劑盒Human insulin-like growth factors binding protein 1,IGFBP-1 ELISA Kit Y3364096T/48T
人粒細(xì)胞巨噬細(xì)胞集落刺激因子(GM-CSF)ELISA試劑盒Human Granulocyte-Macrophage Colony Stimulating Factor,GM-CSF ELISA KitY3364196T/48T
人膠質(zhì)細(xì)胞系來源的神經(jīng)營養(yǎng)因子(GDNF)ELISA試劑盒Human glial cell line-derived neurotrophic factor,GDNF ELISA KitY3364296T/48T
人粒細(xì)胞集落刺激因子(G-CSF)ELISA試劑盒Human Granulocyte Colony Stimulating Factor,G-CSF ELISA Kit Y3364396T/48T
人堿性成纖維細(xì)胞生長因子6(bFGF-6)ELISA試劑盒Human basic fibroblast growth factor 6,bFGF-6 ELISA Kit Y3364496T/48T
人堿性成纖維細(xì)胞生長因子4(bFGF-4)ELISA試劑盒Human basic fibroblast growth factor 4,bFGF-4 ELISA Kit Y3364596T/48T
人酸性成纖維細(xì)胞生長因子1(aFGF-1)ELISA試劑盒Human acidic fibroblast growth factor 1,aFGF-1 ELISA Kit Y3364696T/48T
人凋亡相關(guān)因子配體(FASL)ELISA試劑盒Human Factor-related Apoptosis ligand,FASL ELISA Kit Y3364796T/48T
人凋亡相關(guān)因子(FAS/CD95)ELISA試劑盒Human Factor-related Apoptosis,FAS ELISA Kit Y3364896T/48T
人嗜酸粒細(xì)胞趨化蛋白Eotaxin 1(Eotaxin 1/CCL11)ELISA試劑盒Human Eotaxin 1 ELISA Kit Y3364996T/48T
人內(nèi)分泌腺來源的血管內(nèi)皮生長因子(EG-VEGF)ELISA試劑盒Human Endocrine gland vascular endothelial growth factor,EG-VEGF ELISA Kit Y3365096T/48T
人睫狀神經(jīng)營養(yǎng)因子(CNTF)ELISA試劑盒Human Ciliary Neurotrophic Factor,CNTF ELISA Kit Y3365196T/48T
人CXC趨化因子受體1(CXCR1)ELISA試劑盒Human CXC-chemokine receptor 1,CXCR1 ELISA KitY3365296T/48T
人XC趨化因子受體1(XCR1)ELISA試劑盒Human XC-chemokine receptor 1,XCR1 ELISA KitY3365396T/48T
人二級淋巴組織趨化因子(SLC/CCL21)ELISA試劑盒 Human secondary lymphoid-tissue chemokine,SLC ELISA KitY3365496T/48T
人E鈣粘著蛋白/上皮性鈣黏附蛋白(E-Cad)ELISA試劑盒Human E-Cadherin,E-Cad ELISA Kit Y3365596T/48T
人腦源性神經(jīng)營養(yǎng)因子(BDNF)ELISA試劑盒Human Brain derived neurotrophic facor,BDNF ELISA KitY3365696T/48T
人神經(jīng)調(diào)節(jié)蛋白1(NRG-1)ELISA試劑盒human Neuregulin 1,NRG-1 ELISA KitY3365796T/48T
人多巴胺D2受體(D2R)ELISA試劑盒Human dopamine D2 receptor,D2R ELISA KitY3365896T/48T
人神經(jīng)元凋亡抑制蛋白(NAIP)ELISA試劑盒 Human neuronal apoptosis inhibitory protein,NAIP ELISA KitY3365996T/48T
人食欲素/阿立新B(OX-B)ELISA試劑盒Human Orexin B,OX-B ELISA KitY3366096T/48T
人促睡眠肽(DSIP)ELISA試劑盒Human delta sleep-inducing peptide,DSIP ELISA KitY3366196T/48T
人精氨酸加壓素(AVP)ELISA試劑盒 Human arginine vasopressin,AVP ELISA KitY3366296T/48T
人垂體腺苷酸環(huán)化酶激活肽(PACAP)ELISA試劑盒Human pituitary adenylate cyclase activating polypeptide,PACAP ELISA KitY3366396T/48T
人微管相關(guān)蛋白2(MAP-2)ELISA試劑盒Human microtubule-associated protein 2,MAP-2 ELISA KitY3366496T/48T
人神經(jīng)絲蛋白(NF)ELISA試劑盒Human neurofilament protein,NF ELISA KitY3366596T/48T
人利鉀尿肽(KP)ELISA試劑盒 Human kaliuretic peptide,KP ELISA KitY3366696T/48T
人神經(jīng)降壓素(NT)ELISA試劑盒Human Neurotensin,NT ELISA KitY3366796T/48T
人γ肽(Pγ)ELISA試劑盒Human Peptide γ,Pγ ELISA KitY3366896T/48T
各種類型ELISA測定時(shí),一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。